TF1（人红系白血病细胞）
CBP60808
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| I. General information | |
| Synonyms: | TF1 |
| Background: | The TF-1 cell line was established by T. Kitamura, et al. in October 1987 from a heparinized bone marrow aspiration sample from a 35 year old Japanese male with severe pancytopenia. |
| Species: | Homo sapiens, human |
| Tissue: | bone marrow |
| Disease: | erythroleukemia |
| Gender: | male, 35 years, Japanese |
| Morphology: | lymphoblast |
| Growth Mode: | suspension |
| Doubling Time: | 22 hours |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 13,14 D13S317: 8,9 D16S539: 9,12 D5S818: 13 D7S820: 12 THO1: 7,9 TPOX: 8 vWA: 15,17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+2 ng/ml recombinant human GM-CSF+10%FBS TF1完全培养基，# CBP60808M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | The cells are completely dependent on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth. The cells DO NOT RESPOND to interleukin 5 (IL-5). TF-1 cells respond to a variety of other lymphokines and cytokines such as interleukin 1 (IL-1), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 9 (IL-9),Interleukin 11 (IL-11), interleukin 13 (IL-13), stem cell factor (SCF), leukemia inhibitory factor (LIF) and nerve growth factor (NGF). TF-1 cells do not express glycophorin A or carbonyl anhydrase I. The morphological and cytochemical features, and the constitutive expression of globin genes, indicate the commitment of the cells to the erythroid lineage. Hemin and delta-aminolevulinic acid induce hemoglobin synthesis, and TPA induces dramatic differentiation of the TF-1 cells into macrophage-like cells. The TF-1 cell line is unique because of its responsiveness to multiple cytokines. For more information, please contact Cobioer (4008-750-250). |
