6T-CEM（人T细胞白血病细胞）
CBP60700
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| I. General information | |
| Synonyms: | 6T-CEM |
| Species: | Human |
| Tissue: | Acute lymphocytic leukemia |
| Growth Mode: | suspension |
| Doubling Time: | N/A |
| DNA Profile: | Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI1640+10%FBS 6T-CEM完全培养基，# CBP60700M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | This is a 6-thioguanine resistant variant of CCRF-CEM. It is deficient in hypoxanthine phosphoribosyl transferase (HPRT, HGPRT) and secretes high titers of an immunosuppressive factor. This line may be used as a fusion partner for making human T cell hybridomas. suspensionimmunosuppressive factor Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Handle as potentially biohazardous material under at least Biosafety Level 2 containment. For more information, please contact Cobioer (4008-750-250). |
