NCI-H716（结直肠腺癌细胞）
CBP60737
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| I. General information | |
| Synonyms: | NCI-H716 |
| Background: | Established from the primary tumor of a 33-year-old Caucasian man with cecum adenocarcinoma in 1984; |
| Species: | Homo sapiens, human |
| Tissue: | cecum |
| Disease: | Colorectal adenocarcinoma |
| Gender: | male, 33 years adult,Caucasian |
| Morphology: | epithelial |
| Growth Mode: | suspension, multicell aggregates and some adherent cells |
| Doubling Time: | 40~60hrs |
| DNA Profile: | Amelogenin: X CSF1PO: 11 D13S317: 8,11 D16S539: 11,12 D5S818: 11 D7S820: 10,11 THO1: 6,9.3 TPOX: 8,11 vWA: 16,17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS NCI-H716完全培养基，# CBP60737M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Tumor Formation: | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments: | The cells contain Dopa decarboxylase and, unlike other colorectal lines, contain cytoplasmic dense core granules characteristic of endocrine secretion. The cells do not express the TAG-72 or CA19-9 antigens nor do they produce carcinoembryonic antigen (CEA). This line was derived from cells present in ascites fluid obtained from a patient after treatment with 5-fluorouracil. For more information, please contact Cobioer (4008-750-250). |
