C99（人结肠癌腺癌细胞）
CBP60833
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| I. General information | |
| Synonyms: | C99 |
| Background: | The C99 cell line was established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes' stage C. |
| Species: | human (Homo sapiens) |
| Tissue: | Colon |
| Disease: | Human colorectal adenocarcinoma, moderately well differentiated, Dukes' stage C |
| Gender: | 69-year old male |
| Morphology: | Epithelial |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X CSF1PO: 10,11 D13S317: 8,14 D16S539: 11,13 D5S818: 11,12 D7S820: 9,11 THO1: 8,9.3 TPOX: 8,10 vWA: 16,17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
IMDM+10%FBS C99完全培养基，# CBP60833M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | N/A |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
