RKO-AS45-1（人结肠癌细胞）
CBP60686
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| I. General information | |
| Synonyms: | RKO-AS45-1 |
| Species: | Homo sapiens, human |
| Disease: | Carcinoma |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| DNA Profile: | Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
MEM+10%FBS+1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) RKO-AS45-1完全培养基，# CBP60686M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Oncogene: | p53 + (underexpressed) Ref |
| Genes Expressed: | p53 + (underexpressed) |
| Comments: | The RKO-AS45-1 cell line was generated from the colon carcinoma RKO (ATCC CRL-2577) cell line by transfection with the open reading frame portions of human cDNAs for GADD45 cloned into the expression vector pCMV. The cells contain a stably integrated cytomegalovirus (CMV) promotor driven expression vector. The cells overexpress Gadd45 RNA and protein. The GADD45 gene is p53-regulated and the Gadd45 protein interacts with proteins involved in both cell-cycle regulation and DNA repair.The RKO-AS45-1 cell line has reduced ability to repair U.V.-induced DNA damage.The RKO-AS45-1 cell line can be used together with its parental cell line, RKO (ATCC CRL-2577) to investigate the effects of Gadd45 on DNA repair, apoptosis and drug sensitivity. For more information, please contact Cobioer (4008-750-250). |
