CAL-51（人乳腺癌细胞）
CBP60360
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| I. General information | |
| Synonyms: | CAL-51 |
| Background: | established from the pleural effusion metastasis of a 45-year-old woman with progressive breast adenocarcinoma (after radio-, chemotherapy and surgery) in 1985; rare example of tumor cell line with normal karyotype |
| Species: | human (Homo sapiens) |
| Tissue: | breast |
| Disease: | breast carcinoma |
| Gender: | N/A |
| Morphology: | epithelial-like adherent cells growing in monolayers; the culture contains usually a large amount of cell debris; |
| Growth Mode: | N/A |
| Doubling Time: | 50-60 hours |
| DNA Profile: |
Amelogenin: X CSF1PO: 11,12 D3S1358: 16,17 D5S818: 12,13 D7S820: 7,12 D8S1179: 14 D13S317: 11,13 D16S539: 11,13 D18S51: 15,17 D21S11: 31,32 FGA: 22,23 Penta D: 10,14 Penta E: 7,12 TH01: 7 TPOX: 8,9 vWA:18 |
| Culture Medium: |
DMEM+10%FBS CAL-51完全培养基，# CBP60360M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology: | cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin + |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, LDH, NP |
| Cytogenetics: | human near-diploid karyotype with 28% tetraploidy - 46<2n>XX, no consistent abnormality detected - rare example of tumor cell line with normal karyotype - resembles published karyotype |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
