CAL-148（人乳腺癌细胞）
CBP60359
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| I. General information | |
| Synonyms: | CAL-148 |
| Background: | established from the pleural effusion from a 58-year-old French woman with terminally invasive breast cancer (moderately differentiated ductal adenocarcinoma) after repeated cycles of chemotherapy (including adriamycin and taxol) |
| Species: | human (Homo sapiens) |
| Tissue: | breast |
| Disease: | breast adenocarcinoma |
| Gender: | N/A |
| Morphology: | round to epitheloid cells growing as single cells or as clumps either in suspension or, if supplemented with EGF, in monolayers; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 30-40 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM + 20% FBS+ EGF (0.1 µg/ ml) CAL-148完全培养基，# CBP60359M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human by cytogenetics |
| Cytogenetics: | human near-diploid karyotype with 16% polyploidy - 45-48<2n>XX, +1, +7, -8, -13, +20, add(1)(p36), der(1;16)(q10;p10), del(2)(q11q2?2), del(13)(q1?1q1?3), der(16)t(1;16)(q12-21 - p11) - resembles originator's unpublished karyotype |
| Virus | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
