RWPE-1（人前列腺上皮细胞）
CBP60347
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| I. General information | |
| Synonyms: | RWPE-1 |
| Background: | Epithelial cells derived from the peripheral zone of a histologically normal adult human prostate were transfected with a single copy of the human papilloma virus 18 (HPV-18) to establish the RWPE-1 (ATCC CRL-11609) cell line. Ref |
| Species: | Homo sapiens, human |
| Tissue: | prostate |
| Disease: | normal |
| Gender: | male, 54 years adult, Caucasian, White |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 13 D13S317: 8,14 D16S539: 9,11 D5S818: 12,15 D7S820: 10,11 THO1: 8,9.3 TPOX: 8,11 vWA: 14,18 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
Keratinocyte Serum Free Medium (K-SFM), Kit (GIBCO Catalog Number 17005-042) RWPE-1完全培养基，# CBP60347M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen),Ref (upon exposure to androgen) |
| Receptor Expression: | androgen receptor, expressed , Ref (upregulated upon exposure to androgen) |
| Genes Expressed: | cytokeratin 18, cytokeratin 8, Tumor Supressor Gene(s): p53 +, pRB + Ref |
| Cellular Products: | cytokeratin 18 cytokeratin 8 |
| Tumor Formation: | No, in nude mice (with or without Matrigel) Ref No, in soft agar Ref |
| Comments: | In 3-dimensional Matrigel culture, RWPE-1 cells organize into acini and secrete PSA into the lumen when exposed to androgen. Ref When injected with Matrigel or with stromal cells, into male athymic rodents, RWPE-1 cells also organize into acini Ref and produce PSA. Cells from the RWPE-1 cell line were further transformed by Ki-ras using the Kirstin murine sarcoma virus (Ki-MuSV) to establish the tumorigenic RWPE-2 cell line (ATCC CRL-11610) Ref and the RWPE2-W99 (ATCC CRL-2853) cell line. Further, a family of tumorigenic cell lines, that mimics multiple steps in prostate cancer progression, was also derived from RWPE-1 cells by exposure to N-methyl-N-nitrosourea (MNU). See the WPE1-NA22 (ATCC CRL-2849), WPE1-NB14 (ATCC CRL-2850, WPE1-NB11 (ATCC CRL-2851) and WPE1-NB26 (ATCC CRL-2852) cell lines. The depositor reports that the RWPE-1 cell line (ATCC CRL-11609) was screened, and found negative for, Hepatitis B virus, Hepatitis C virus and Human immunodeficiency virus. ATCC confirmed this cell line is positive for the presence of HPV viral DNA sequences via PCR. For more information, please contact Cobioer (4008-750-250). |
