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APOE/TCR Activator/CHO

CBP74107

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I. Background
APOE include Lipoprotein Glomerulopathy and Hyperlipoproteinemia, Type Iii. Among its related pathways are Neuroscience and Lipoprotein metabolism. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and signaling receptor binding.
 
II. Introduction
Host Cell: CHO
Expressed gene: APOE
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): Apolipoprotein E
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: F12K+10%FBS+600ug/ml hygromycin+2ug/ml puromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
III. Description of Host Cell Line
Organism: Cricetulus griseus, hamster, Chinese
Tissue: Ovary
Disease: Hamster Chinese ovary
Morphology: Epitheloid cell
Growth Properties: Adherent
 
IV. Representative Data

Figure 1. Recombinant CHO-cells constitutively expressing  APOE and an engineered T cell receptor (TCR) activator.

 

Figure 2. Dose response of LILRB4 Blocking antibody in LILRB4 Effector Reporter Cells(C5) With APOE TCR Activitor CHO Cells, the EC50= 40 ng/ml.

 

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