WSU DLCL2（人淋巴瘤细胞）
CBP60273
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| I. General information | |
| Synonyms: | WSU DLCL2 |
| Background: | derived from the pleural effusion of a 41-year-old Caucasian man with B-cell non-Hodgkin lymphoma (B-NHL, diffuse large cell lymphoma non-cleaved type, intermediate grade - transformed from follicular small cleaved cell, low grade) at the status of relapse/progressive disease in 1990; cell line carries EZH2 Y641F mutation |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | B cell lymphoma |
| Gender: | N/A |
| Morphology: | round and oval cells growing singly or in small clumps in suspension; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 50-70 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS WSU DLCL2完全培养基，# CBP60273M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD 138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image |
| Fingerprint: | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Species: | confirmed as human by cytogenetics and species PCR |
| Cytogenetics: | human hyperdiploid karyotype - 47(43-48)<2n>XY, +7, +8, t(1;2)(p36;q37), del(3)(q25), t(4;14)(q27;q31), der(7)t(3;7)(q25;p13), i(7p), add(8)(q24)x2, del(13)(?q21?q22), t(14;18)(q32;q21), del(15)(q25q27) - carries t(14;18) effecting BCL2-IGH fusion - matches published karyotype |
| Virus | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
