L-363（人多发性骨髓瘤细胞）
CBP60240
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| I. General information | |
| Synonyms: | L-363 |
| Background: | established from the peripheral blood of a 36-year-old woman with plasma cell leukemia (IgG) in 1977; cells were described in the literature to be EBNA-negative and to express mRNA for proto-oncogene BCL2; plasma cell leukemia is related to multiple myeloma |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | plasma cell leukemia |
| Gender: | N/A |
| Morphology: | single, large, round or oval cells in suspension; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 25 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS L-363完全培养基，# CBP60240M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, cyCD79a -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, LDH, PEP B |
| Cytogenetics: | human hyperdiploid karyotype with 11% polyploidy - 49(45-50)<2n>X, -X, -X, -6, +7, +8, +11, +11, +14, +19, -22, t(5;8)(q12/13;q24.32), der(8)(t(5;8)(q12/13;q24.32), der(8)t(7;8)(q11.2;p22), del(11)(q13)x2, add(14)(q32), del(17)(p12), add(19)(q13) - sideline with add(13)(q34) - 11q13 and 14q32 breakpoints recurrent in PCL - resembles published karyotype |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
