U-2 OS（人骨肉瘤细胞）
CBP60238
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| I. General information | |
| Synonyms: | U-2 OS |
| Background: | Cell line derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl. |
| Species: | human (Homo sapiens) |
| Tissue: | bone |
| Disease: | Human Osteosarcoma |
| Gender: | a 15 year old girl |
| Morphology: | N/A |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X CSF1PO: 13 D13S317: 13 D16S539: 11,12 D5S818: 11 D7S820: 11,12 THO1: 6,9.3 TPOX: 11,12 vWA: 14,18 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
McCoy's 5a+10%FBS U-2 OS完全培养基，# CBP60238M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Not specified |
| Subculture Routine: | Split subconfluent 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks. |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
