SNU-423（人肝癌细胞）
CBP60216
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| I. General information | |
| Synonyms: | SNU-423 |
| Background: | SNU-423 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus doxorubicin. Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum. |
| Species: | Homo sapiens, human |
| Tissue: | liver |
| Disease: | grade III/IV, pleomorphic hepatocellular carcinoma |
| Gender: | male, 40 years, Asian |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | 72 hrs |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 10,13 D16S539: 9 D5S818: 10 D7S820: 12 THO1: 6,9 TPOX: 8 vWA: 15 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS SNU-423完全培养基，#CBP60216M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | Blood Type B; Rh + |
| Comments: | Grossly, the original tumor was single nodular with perinodular extensions. Histologically, it was trabecular type. The cultured cells are multinucleated. Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed. For more information, please contact Cobioer (4008-750-250) |
