NCI-H69（人小细胞肺癌细胞）
CBP60180
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| I. General information | |
| Synonyms: | NCI-H69 |
| Species: | Homo sapiens, human |
| Tissue: | lung |
| Disease: | carcinoma; small cell lung cancer |
| Gender: | male, 55 years adult, Caucasian |
| Morphology: | floating aggregates |
| Growth Mode: | suspension, multicell aggregates |
| DNA Profile: | CSF1PO: 10, 12 D13S317: 12 D16S539: 11 D5S818: 11, 13 D7S820: 9 THO1: 8, 9 TPOX: 10 vWA: 16, 17 Amelogenin: XY Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+20%FBS NCI-H69完全培养基，# CBP60180M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Receptor Expression: | insulin-like growth factor II (IGF II) |
| Oncogene: | myc +; myb +; fes +; fms +; raf +; ras + |
| Genes Expressed: | Oncogenes: myc +; myb +; fes +; fms +; raf +; ras + |
| Tumor Formation: | Yes, in nude mice (The cells form tumors with typical small cell carcinoma histology.) Yes, forms colonies in soft agar |
| Comments: | This cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics. The cells grow in aggregates, thus cell counts are not accurate. The cells stain positively for cytokeratins. The line can be adapted to grow in shaker flask or spinner flask systems. The N-myc gene is amplified, and there is expression of the mRNA and protein. C-myc mRNA, but not protein, is expressed at a low level. There is expression of c-myb, v-fes, v-fms, c-raf 1, Ha-ras, Ki-ras and N-ras mRNA. For more information, please contact Cobioer (4008-750-250). |
