NCI-H522（人非小细胞肺癌细胞）
CBP60140
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| I. General information | |
| Synonyms: | NCI-H522 |
| Background: | This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lung cancer obtained from a patient prior to therapy. |
| Species: | Homo sapiens, human |
| Tissue: | lung |
| Disease: | stage 2,adenocarcinoma; non-small cell lung cancer |
| Gender: | male, 58 years, Caucasian, The patient was a smoker (60-pack-year). |
| Morphology: | epithelial |
| Growth Mode: | adherent, single cells and loosely attached clusters |
| DNA Profile: | Amelogenin: X CSF1PO: 10 D13S317: 10 D16S539: 12 D5S818: 11 D7S820: 9,10 THO1: 7,9.3 TPOX: 9,10 vWA: 17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS NCI-H522完全培养基，# CBP60140M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | The cells carry a mutation in codon 191 of the TP53 gene. The cells are not able to synthesize the peptide neuromedin B (NMB) or the gastrin releasing peptide (GRP). The cells stain positive for keratin and vimentin but are negative for neurofilament triplet protein. For more information, please contact Cobioer (4008-750-250). |
