NCI-H23（人非小细胞肺癌腺癌细胞）
CBP60132
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| I. General information | |
| Synonyms: | NCI-H23 |
| Background: | This line was derived from a lung cancer obtained from a patient prior to therapy. |
| Species: | Homo sapiens, human |
| Tissue: | lung |
| Disease: | adenocarcinoma; non-small cell lung cancer |
| Gender: | male, 51 years, Black |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | 38 hrs |
| DNA Profile: | Amelogenin: X CSF1PO: 10 D13S317: 12 D16S539: 11 D5S818: 12,13 D7S820: 9,10 THO1: 6 TPOX: 8,9 vWA: 16,17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS NCI-H23完全培养基，# CBP60132M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Oncogene: | myc +; src +; abl +; erb +; ras +; sis - |
| Genes Expressed: | myc +; src +; abl +; erb +; ras +; sis - |
| Comments: | The cells carry the K-ras 12 mutation, and there is a mutation in codon 246 (ATC -> ATG, isoleucine -> methionine) of the p53 gene. There is expression of C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras and N-ras RNAs. The cells express heterogeneous mRNA expression for PDGF A and B chain, transforming growth factor alpha and beta and the epidermal growth factor receptor (EGFR). NCI-H23 exhibits a high degree of c-myc DNA amplification (20-fold) but no detectable amplification of c-myc RNA. The cells stain positive for keratins 5+8 and 18 and vimentin but are negative for neurofilament. NCI-H23 cells are L-dopa decarboxylase-negative. They have a reported colony forming efficiency of 9.7% in soft agarose. For more information, please contact Cobioer (4008-750-250). |
