HCC-78（人肺癌细胞（非小细胞））
CBP60100
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| I. General information | |
| Synonyms: | HCC-78 |
| Background: | established from the pleural effusion of a 65-year-old man with adenocarcinoma of the lung, typed as non-small cell lung carcinoma; cells are described in the literature to carry a ROS1 rearrangement; matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-78BL) |
| Species: | human (Homo sapiens) |
| Tissue: | lung |
| Disease: | non-small cell lung carcinoma |
| Gender: | N/A |
| Morphology: | large epitheloid cells growing in monolayers and aggregates; after trypsination cells are not always dispersed as single cells; image; image |
| Growth Mode: | N/A |
| Doubling Time: | ca. 60-70 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS HCC-78完全培养基，# CBP60100M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 (+), cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin + |
| Fingerprint: | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Species: | confirmed as human by PCR speciation and cytogenetics |
| Cytogenetics: | human flat-moded complex hypotetraploid karyotype with 6% polyploidy - 80-91<4n>XYY/XXYY, add(X)(q25)x2, add(1)(?p11), add(4)(p15), add(4)(q31), add(16)(q23), der(16)t(1;16)(p12;q23)x2, dup(16)(q11q13)add(16)(q13), add(19)(q13)x2, inc. |
| Virus | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
