LOU-NH91（人肺癌鳞癌细胞）
CBP60081
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| I. General information | |
| Synonyms: | LOU-NH91 |
| Background: | established from the lower right lung lobe removed two months after diagnosis of highly differentiated squamous cell lung carcinoma with metastasis to one lymph node (no visceral involvement) in a 64-year-old woman (without any prior therapy) |
| Species: | human (Homo sapiens) |
| Tissue: | lung |
| Disease: | lung squamous cell carcinoma |
| Gender: | N/A |
| Morphology: | adherent ovoid-to-epitheloid cells growing in monolayers; image; image |
| Growth Mode: | N/A |
| Doubling Time: | ca. 80 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640 + 20% FBS LOU-NH91完全培养基，# CBP60081M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin + |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, LDH, NP |
| Cytogenetics: | human hyperdiploid karyotype with declining hyperhaploid sideline (2%) and 9% polyploidy - 50-53<2n>XX/XXXX, +5, +5, +7, +7, -13, +17, -18, +22, +22, +1-2mar, del(4)(q27), ?add(22)(q13)x2 (major clone) - 54<2n>XXXX, +5, +5, +7, +7, +22, +22 (minor clone) - 27XX, +5, +7, +22 - matches published karyotype - unstable karyotype with recent gain of structural rearrangements |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
