HTC-C3（人甲状腺癌细胞）
CBP61200
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| I. General information | |
| Synonyms: | HTC-C3 |
| Background: | established from cancer cells disseminated in the pleural fluid of a 44-year-old woman with undifferentiated giant cell carcinoma of the thyroid after chemotherapy, x-ray hyperthermia and OK432 injections in 1986; cells were described in the literature to contain parathyroid-like activity |
| Species: | human (Homo sapiens) |
| Tissue: | thyroid |
| Disease: | thyroid carcinoma |
| Gender: | N/A |
| Morphology: | epithelial-like cells growing as monolayer |
| Growth Mode: | N/A |
| Doubling Time: | ca. 100 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM+20%FBS HTC-C3完全培养基，# CBP61200M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, GFAP -, neurofilament -, thyroglobulin -, vimentin - |
| Fingerprint: | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Species: | confirmed as human by cytogenetics and species PCR |
| Cytogenetics: | human near-triploid karyotype with 23% polyploidy - 68(68-73)<3n>XXXXX, add(X)(p11), add(X)(p11)add(X)(q11), add(X)(q11), del(X)(p11), der(6)t(X;6)(q23;q25), inc. |
| Virus | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
