HCC44（人非小细胞肺癌细胞）
CBP61182
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| I. General information | |
| Synonyms: | HCC44 |
| Background: | established from the lung of a 54-year-old woman with non-small cell lung cancer of the adenocarcinoma type |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | non-small cell lung carcinoma |
| Gender: | N/A |
| Morphology: | adherent epitheloid cells growing as monolayer; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 30 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS HCC44完全培养基，# CBP61182M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with sparfloxacin, then negative in microbiological culture and PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin + |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human by cytogenetics |
| Cytogenetics: | human flat-moded hyperpentaploid karyotype with 8% polyploidy - 120-124<5n>XX, +1, +1, +3, +3, -4, -4, -5, +7, +7, +7, -9, -9, -10, +11, -18, -21, -21, +22, +22, +22, +7-14mar, der(1)t(X;1)(q11;p11)x2, del(1)(q21)x2, i(1q), t(1;3)(p11;q11), add(2)(q?11), del(2)(p11), add(3)(q28), add(3)(p11), i(5p), add(8)(p2?), add(8)(q2?), add(8)(q24), der(9;19)(q10;q10)x2, add(10)(q2?5), add(11)(p13), der(11)add(11)(p1?1)add(11)(q25), add(12)(p13), add(16)(p11), add(17)(p1?2), add(18)(p11), del(20)(q12)x1-2, add(20)(q13)x2 - 2-6 dmin present in 80% cells |
| Virus | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
