CF-1 MEF（小鼠胚胎成纤维细胞）
CBP61128
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| I. General information | |
| Synonyms: | CF-1 MEF |
| Background: | The cell line was established by ATCC in 2003 from embryonic day 14 (E14) CF-1 mouse embryos. |
| Species: | Mus musculus, mouse |
| Tissue: | Embryo |
| Disease: | N/A |
| Gender: | 14 days gestation embryo， male and female mixed |
| Morphology: | Fibroblast |
| Growth Mode: | adherent |
| Doubling Time: | N/A |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM+10%FBS+ 1%Non Essential Amino Acids (NEAA) CF-1 MEF完全培养基，# CBP61128M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | N/A |
| Receptor Expression: | N/A |
| Oncogene: | N/A |
| Genes Expressed: | N/A |
| Cellular Products: | N/A |
| Tumor Formation: | N/A |
| Comments: | The growth of these cells should be arrested before being used as a feeder layer. ATCC has successfully irradiated (SCRC-1040.1™) and treated the cells with Mitomycin C (SCRC-1040.2a™) for use as a feeder layer. If the MEFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no. 6 (P6). For more information, please contact Cobioer (4008-750-250). |
