MDBK（牛肾细胞）
CBP61030
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| I. General information | |
| Synonyms: | MDBK |
| Background: | The MDBK cell line was derived from a kidney of an apparently normal adult steer, February 18, 1957, by S.H. Madin and N.B. Darby. The cells were originally obtained in July, 1967 at passage 96 from the ATCC as a frozen ampule (F-145). |
| Species: | Bos taurus, cow |
| Tissue: | kidney |
| Gender: | male, adult |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Culture Medium: |
MEM+10%HS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) MDBK完全培养基，# CBP61030M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Genes Expressed: | keratin |
| Comments: | Vesicular stomatitis, Orsay (Indiana) Infectious bovine rhinotracheitis Bovine parvovirus Bovine adenovirus 2 Bovine adenovirus 3 Bovine viral diarrhea virus 1 , Bovine viral diarrhea virus 1 Parainfluenza 3 poliovirus 2 A specific lot of CCL-22, MDBK (NBL-1) bovine kidney cells was found to be positive for bovine viral diarrhea virus (BVDV), following an investigation by ATCC (Dec. 2015). Testing for BVDV was performed in compliance with the Code of Federal Regulations, Title 9, Section 113.52 (E &F) using virus-specific fluorescent antibody (FA) technique as well as non-specific tests for hemadsorption and cytopathic effects (CPE). Samples were found to be positive for BVDV by FA. Hemadsorption and CPE were not observed in the sample inoculated cultures. As a result, the BSL status for CCL-22 was changed to “2”. Subsequent lots of CCL-22, MDBK (NBL-1) bovine kidney cells are tested for BVDV and the results will be indicated on the Certificate of Analysis for the lot. The cells are positive for keratin by immunofluorescence For more information, please contact Cobioer (4008-750-250). |
