SK-N-BE(2)（人神经母细胞瘤细胞）
CBP60743
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| I. General information | |
| Synonyms: | SK-N-BE(2) |
| Background: | The SK-N-BE(2) neuroblastoma cell line was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy. |
| Species: | Homo sapiens, human |
| Tissue: | brain; derived from metastatic site: bone marrow |
| Disease: | neuroblastoma |
| Gender: | male, 2 years |
| Morphology: | neuroblast |
| Growth Mode: | mixed, adherent and suspension |
| Doubling Time: | 30 hrs |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 10 D13S317: 11 D16S539: 9,11 D5S818: 12 D7S820: 9,10 THO1: 6,7 TPOX: 8,11 vWA: 18 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
MEM:F12(1:1)+10%FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) SK-N-BE(2)完全培养基，# CBP60743M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Tumor Formation: | Yes, an inoculum of 107 cells produced tumors in cortisonized hamster cheek pouches with 18% frequency |
| Comments: | The cells exhibit moderate levels of dopamine beta hydroxylase activity. SK-N-BE(2) cells have a reported saturation density greater than 1 X 106 cells/cm2. The morphology of the cells varies with some cells having long processes and others that are epithelioid like. The cells will aggregate, form clumps and float. For more information, please contact Cobioer (4008-750-250). |
