Lec1（仓鼠卵巢细胞）
CBP60862
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| I. General information | |
| Synonyms: | Lec1 |
| Background: | This line is a mutant clone derived from the parental CHO clone Pro-5 (a proline auxotroph, see ATCC CRL-1781) by selection for resistance to wheat germ agglutinin. |
| Species: | Cricetulus griseus, hamster, Chinese |
| Tissue: | ovary |
| Gender: | female |
| Morphology: | epithelial |
| Growth Mode: | loosely adherent |
| Culture Medium: |
Alpha-MEM ribonucleosides and deoxyribonucleosides+10%FBS Lec1完全培养基，# CBP60862M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | The cells lack GlcNAc glycosyl transferase so that N-linked carbohydrates are blocked at the Man5-GlcNAC2-Asn intermediate. The line may be useful for studying the effects of altered N-linked glycosylation on the function and compartmentalization of endogenous glycoproteins or glycoproteins introduced by viral infection or transfection of foreign DNA. The population contains Pro+ revertants at low frequency (approximately 1 in 100,000 cells), and should be suitable for complementation studies which require the Pro- marker. The cells are proline auxotrophs and must be grown in a medium that contains proline (40 mg/L). The population contains Pro+ revertants at low frequency (approximately 1 in 100000 cells), and should be suitable for complementation studies which require the Pro- marker. For more information, please contact Cobioer (4008-750-250). |
