647-V（人恶性2级膀胱癌细胞）
CBP60831
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| I. General information | |
| Synonyms: | 647-V |
| Background: | established from a male patient with a primary urothelial bladder carcinoma (malignant, grade 2) |
| Species: | human (Homo sapiens) |
| Tissue: | bladder |
| Disease: | urothelial bladder carcinoma |
| Gender: | N/A |
| Morphology: | adherent epithelial-like cells growing in monolayers |
| Growth Mode: | N/A |
| Doubling Time: | ca. 30 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM+10%FBS 647-V完全培养基，# CBP60831M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, NP |
| Cytogenetics: | human flat-moded hyperdiploid/hypotriploid karyotype with 12% polyploidy - 52-60<3n>XX, -X/Y, -2, -4, -6, -9, -10, -12, -12, -14, -15, -16, -17, -18, +5mar, del(1)(p32), del(2)(q23)/add(2)(q2?), add(3)(q27), add(3)(q1?), i(5p), i(6p), i(7p)x2, add(8)(q24), del(11)(q22q24), del(13)(q?11q?14), der(19)t(12;19)(q13;q13), add(21)(p11) - i(5p) associated with bladder cancer |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
