WILL-2（人B细胞淋巴瘤）
CBP60624
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| I. General information | |
| Synonyms: | WILL-2 |
| Background: | established in 2008 post-mortem from the ascites of a 63-year-old Japanese woman with diffuse large B-cell lymphoma after relapse |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | B cell lymphoma |
| Gender: | N/A |
| Morphology: | small and mostly single round cells growing in suspension; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 36-48 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+20%FBS WILL-2完全培养基，# CBP60624M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 (+), CD38 +, CD138 +, HLA-DR +; |
| Fingerprint: | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile |
| Species: | N/A |
| Cytogenetics: | human near-diploid karyotype; 46(43-47)<2n>XX, del(X)(q23), dup(1)(q21q32), ins(1;X)(q21;q27q23), t(4;17)(p15;q12). t(5;9)(q14;p21), t(8;22)(q24;q11), t(14;18)(q32;q21); carries t(8;22) rearrangement effecting IGL-MYC, and t(14;18) rearrangements effecting IGH-BCL2; resembles published karyotype |
| Molec. Genetics: | expression of fusion gene IGH-BCL2 confirmed by RT-PCR |
| Virus | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
