LN-18（人脑胶质母细胞瘤）
CBP60595
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| I. General information | |
| Synonyms: | LN-18 |
| Background: | The LN-18 cell line was established in 1976 from cells taken from a patient with a right temporal lobe glioma. |
| Species: | Homo sapiens, human |
| Tissue: | brain/cerebrum; right temporal lobe |
| Disease: | grade IV,glioblastoma; glioma |
| Gender: | White,male, 65 years |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 12 D13S317: 12,13 D16S539: 11,13 D5S818: 11,13 D7S820: 8,10 THO1: 9 TPOX: 8 vWA: 17,18 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM+10%FBS LN-18完全培养基，# CBP60595M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | HLA A2, A9, B5, BW35, DRW3,Ref |
| Oncogene: | p53 + (mutated, TGT (Cys) --> TCT (Ser) mutation at codon 238), PTEN + (wild type), p16 - (deleted), p14ARF - (deleted) Ref |
| Genes Expressed: | fibronectin,Ref |
| Cellular Products: | fibronectin,Ref |
| Tumor Formation: | Yes, forms tumors in nude mice |
| Comments: | The cells are negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells exhibit mutated p53 (TP53) and possible homozygous deletions in the p16 and p14ARF tumor suppressor genes. They have a wild-type PTEN gene. Stimulation of the cells with Fas ligand lead to apoptotic cell death within 16 hours. The cells were also killed by puromycin in a dose dependent manner.Bcl-2 protects these cells from Fas ligand-induced cell death but was shown to have only a small protective effect on puromycin-induced apoptosis. For more information, please contact Cobioer (4008-750-250). |
