HPAC（人胰腺癌细胞）
CBP60539
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| I. General information | |
| Synonyms: | HPAC |
| Background: | HPAC is a pancreatic adenocarcinoma epithelial cell line derived in 1985 from a nude mouse xenograft of a primary tumor removed from the head of the pancreas of a woman with moderate to well differentiated pancreatic adenocarcinoma of ductal origin. |
| Species: | Homo sapiens, human |
| Tissue: | pancreas |
| Disease: | adenocarcinoma |
| Gender: | Caucasian, female, 64 years |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | 41 hrs |
| DNA Profile: | Amelogenin: X CSF1PO: 13 D13S317: 11 D16S539: 9,10 D5S818: 12 D7S820: 10,12 THO1: 9.3 TPOX: 10,11 vWA: 15,17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM/F12(1:1)+0.002 mg/ml insulin+0.005 mg/ml transferrin+ 40 ng/ml hydrocortisone+ 10 ng/ml epidermal growth factor +10%FBS HPAC完全培养基，#CBP60539M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Receptor Expression: | epidermal growth factor (EGF), expressed，glucocorticoid, expressed，epidermal growth factor (EGF); glucocorticoid |
| Genes Expressed: | HPAC cells are positive for keratin and negative for vimentin and chromogranin A. |
| Tumor Formation: | Yes, the cells form tumors in athymic nude mice at the site of inoculation which are histologically similar to the tumor of origin，Yes, the cells have a colony forming efficiency of 64% on plastic and 3.2% in agarose. |
| Comments: | HPAC proliferation is stimulated by insulin, insulin-like growth factor I (IGF-I), epidermal growth factor (EGF), and transforming growth factor alpha (TGF alpha). Cell growth is suppressed by dexamethasone and other glucocorticoids. Crude HPAC cell exacts contained significant concentrations of tumor-associated antigens CEA, CA 125, and CA 19-9. In culture, HPAC cells form monolayers of morphologically heterogenous polar epithelial cells. HPAC is the first reported human pancreatic adenocarcinoma cell line to express a functional glucocorticoid receptor. Immunohistochemical staining revealed that HPAC cells express the pancreatic ductal epithelium marker DU-PAN-2 as well as antigens recognized by the monoclonal antibodies HMFG1 and AUA1. For more information, please contact Cobioer (4008-750-250). |
