KRAS G12V/BaF3
CBP73230
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| I. Introduction | |
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Cell Line Name： |
KRAS G12V/BaF3 |
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Host Cell: |
Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Application: | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Freeze Medium: | 90% FBS+10% DMSO |
| Complete Culture Medium: | RPMI-1640+10%FBS+1ug/ml puromycin |
| Mycoplasma Status: | Negative |
| II. Background | |
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Human KRAS also known as C-K-RAS, CFC2, and K-RAS2A, GenBank Accession No. NM_033360, a.a. 2-185, with G12V mutation and N-terminal His-tag, and expressed in a Baculovirus infected Sf9 cell expression system. MW=22 kDa. |
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| III. Representative Data | |
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1..WB of KRAS G12V/BaF3
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2.Anti-proliferation assay
Figure 3. Anti-proliferation assay of two reference compounds on the KRAS G12V/BaF3 Stable Cell Line. |
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