GLP1R/CRE-Luc/HEK293
CBP71117
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| I. Background | |
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GLP-1R 与其他四种胰高血糖素受体（GCGR、GLP-2R、GIPR 和 GHRHR）一起属于 促胰液素 (B1 类) GPCR 家族，其内源性配体是肽激素。 B1 类 GPCRs 在 N 端具有一个较 大的、结构保守的胞外结构域（ECD），由 120-160 个残基组成，形成三层 α-β-β-α 折叠， 并由三个层间二硫键稳定。 |
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| II. Description | |
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GLP1R CRE-Luc HEK293 报告基因药靶模型很好的模拟了体内 GLP1R 的信号转导过程，原理见下图所示。
Figure 1. GLP1R CRE-Luc HEK293 细胞模型原理图
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| III. Introduction | |
| Host Cell: |
HEK293 |
| Expressed gene: | Genbank Accession Number NM_002062; no expressed tags |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): |
GLP-1 |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+400ug/ml G418 |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for GLP1R receptor |
| Transducer: | Gs |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| V.Representative Data | |
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Figure 3. GLP1R CRE-Luc HEK293 Assay 验证结果。 |
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