C3aR/CHO
CBP71375
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| I. Background | |
| C3aR binds the complement activation fragment, C3a. It is a G-protein coupled receptor of 482 amino acids, notable for its large second extracellular loop. As with the other closely related anaphylatoxin receptors, the genetic locus for this receptor consists of two exons separated by a large intron. The receptor is widely expressed, and in high levels on cells of the myeloid lineage. | |
| II. Introduction | |
| Host Cell: |
CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5ug/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for C3aR receptor |
| Transducer: | Gi |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| Ⅳ. Representative Data | |
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Figure 1. Recombinant C3aR/CHO constitutively expressing C3aR. |
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Figure 2. HTRF cAMP Assay With C3aR CHO Cells (C4). |
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