PVRIG(CD112R) Effector Reporter Cell
CBP74120
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| I. Background | |
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PVRIG (CD112R) is an regulatory protein expressed on T-cells which inhibits immune activation when binding to its counterpart on APCs, PVRL2 (CD112, Nectin-2). Since both PVRL2 and PVR can activate DNAM-1 (CD226), PVRIG works synergistically with and separately from TIGIT. PVRL2 is overexpressed in a variety of tumor types, including breast and ovarian cancers, suggesting that its interaction with PVRIG is a major mechanism of immune suppression in those diseases. |
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| II. Description | |
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Human secreted CD112R, also known as Poliovirus Receptor-related Immunoglobulin Domain
containing Protein (PVRIG), GenBank Accession No. NM_024070, a.a. 41-172, with C-terminal Fc-Fusion-Avi
tag, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using AviTag technology. MW = 42 kDa.
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| III. Introduction | |
| Expressed gene: |
PVRIG(CD112R) |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | Poliovirus Receptor-related Immunoglobulin Domain-containing Protein, Transmembrane Protein PVRIG, 79270-1, 79270-2 |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
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Figure 1. Dose Response of PVRIG Blocking Antibody in PVRIG(CD112R) Effector Reporter Cells (Clone 7) With CD112/TCR Activator - CHO Cells. |
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