PDL1/Raji
CBP74095
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| I. Background | |
| The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T-cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. The PD-1 ligands are found on most cancers, and PD-1:PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes. | |
| II. Description | |
| Recombinant Raji cells expressing human PD-L1 (Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7- H1), GenBank accession # NM_014143). | |
| III. Introduction | |
| Host Cell: | Raji |
| Expressed gene: | PDL1 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | Programmed Cell Death 1 Ligand 1, PDL1, PD-L1, B7 homolog 1, B7H1, B7-H1, CD274, PDCD1L1 |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+200ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Binding Assay,FACS |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | lymphoblast |
| Disease: | Burkitt's lymphoma |
| Morphology: | lymphoblast |
| Growth Properties: | suspension |
| Ⅴ. Representative Data | |
Figure 1.RecombinantRajicells constitutively expressing human PD-L1 (Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7- H1), GenBank accession #NM_014143).
Figure 2. Blocking of PD-L1 induced PD-1/SHP2 Reporter Cells (C17) Activity by PD-L1 Blocking Ab with PD-L1 Target cells (suspended).
Figure 3. Blocking of PD-1 induced PD-1/SHP2 Reporter Cells (C17) Activity by PD-1 Blocking Ab with PD-L1 Target cells (suspended).
