Package-Ref™ Human Methylation Reference Standard II
CBPE0390
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产品描述
产品数据库
| Cat No. | Name | Average Methylation Level | Description |
| CBPE0390-1 | Human Non-Methylation Reference Standard | Average Methy=0% | 本产品通过全基因扩增(WGA)得到，可作为DNA甲基化分析的阴性对照，检验方法采用RRBS、ddPCR法和酶切法验证，酶切法采用McrBc或者MspI和HpaII对样本进行酶切。样本来源于细胞系，更接近真实样本。 |
| CBPE0390-2 | Human Low-Methylation Reference Standard | Average Methy <30% | 产品通过在细胞层面敲除DNMT3A和DNMT3B蛋白，提取gDNA得到，检验方法采用RRBS、WGBS和酶切法验证，酶切法采用McrBc或者MspI和HpaII对样本进行酶切。样本来源于细胞系，更接近真实样本。 |
| CBPE0390-3 | Human Medium-Methylation Reference Standard | 30% |
本产品通过在细胞上过表达DNMT3A和DNMT3B蛋白，提取gDNA得到，检验方法采用RRBS、WGBS和酶切法验证，酶切法采用McrBc或者MspI和HpaII对样本进行酶切。样本来源于细胞系，更接近真实样本。 |
| CBPE0390-4 | Human High-Methylation Reference Standard | Average Methy＞70% |
本产品为天然样本，检验方法采用RRBS、WGBS和酶切法验证，酶切法采用McrBc或者MspI和HpaII对样本进行酶切。样本来源于细胞系，更接近真实样本。 |
| CBPE0390-5 | Human Hyper-Methylation Reference Standard | Average Methy≈100% | 本产品的全基因组甲基化DNA 是通过甲基转移酶（M.SssI）修饰人的基因组 DNA 而得，可以作为DNA甲基化分析的阳性对照，检验方法采用RRBS、ddPCR法和酶切法验证，酶切法采用McrBc或者MspI和HpaII对样本进行酶切。样本来源于细胞系，更接近真实样本。 |
| Remark | 1. MspI可酶切非甲基化和甲基化的DNA，对甲基化不敏感。 | ||
| 2. HpaII对CpG甲基化敏感，不能酶切甲基化的DNA，只能酶切非甲基化的DNA。 | |||
| 3. 本产品均采用同种细胞系 | |||
| Representative Data | |||
|
Figure 1. DNA methylation analysis by Reduced representation bisulfite sequencing (RRBS) on different samples.
Figure 2. Different samples digested by MspI or HapII. |
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| Product Information | |||
| Intended Use | Research Use Only | ||
| Concentration | Download for COA | ||
| Purity | Download for COA | ||
| DNA electrophoresis | Download for COA | ||
| Storage | Download for COA | ||
| Expiry | Download for COA | ||
| Remark | The data in the instruction manual is the data of a single test, and the specific data of each batch is subject to delivery. | ||
